Capto sp impres pdf download

Capto sp impres capto q impres suits largescale purification processes capto sp impres is a bioprocess chromatography resin developed for largescale purification of biopharmaceutical proteins. Capto mmc impres can be used in the initial polishing step in mab purification processes, as well as for polishing of antibody fragments such as domain. Predictor capto adhere impres filter plates, 96well plates for highthroughput screening or process development. Capto adhere impres multimodal chromatography resin. The strong ion exchange ligand groups of capto sp impres left. Isoelectric point pi and aggregate content for mabs used in the studies with capto s impact mab pi aggregate content mab a 8. General description capto sp impres is a strong cation exchange bioprocess medium, designed to meet the demands of modern largescale manufacturers for fast, efficient, and costeffective intermediate and polishing protein purification application capto sp impres is a strong cation exchanger for the highthroughput intermediate purification and polishing steps of a wide range of.

Based on the wellestablished capto base matrix and with traditional ionic group ligands, it provides many opportunities for improved productivity and straightforward process development. Pdf purification of antibody fragments for the reduction of charge. Capto sp impres pdf capto sp impres ge healthcare, pack of 25 ml. Pdf recently, antibody fragments have been studied as therapeutic agents because they lack fc effector. Capto sp impres capto q impres process development forum. Capto q impres is a bioprocess chromatography resin developed for largescale purification of biopharmaceutical proteins. Capto sp impres ion exchange chromatography resin enjoy free shipping on most orders placed online terms apply with a high binding capacity and small bead size, capto sp impres strong cation exchanger is a good choice for reliable and robust polishing steps in industrial purification. Capto sp impres strong cex was used in the mab x purification process to remove. Capto sp impres and capto q impres are strong cation and strong anion exchangers for the highthroughput intermediate. Capto q and capto s are strong ion exchange media for packed bed chromatography that increase speed and. Capto sp impres is a sulfonate group while the ligand of capto q impres is a quaternary amine group fig 2.

Ion exchange chromatography capto q impres, capto sp impres, capto s impact 7 capto q, capto s, capto deae 8 affinity chromatography capto heparin 8 mabselect prisma, mabselect 9 mabselect xtra, mabselect sure, mabselect sure lx, capto l 10 viii select 10 kappaselect, lambda fabselect, igg select, capto blue, capto blue high sub, capto chelating, vii select, captodevirs, and alpha. Packing capto impres resins with verified packing methods. The sp impres resin and ph gradient exhibited the most effective separation potency using. The separation was also performed with a ph gradient using a capto s and b sp impres. Scaling up allows for high resolution combined with quick flow rates. The rigidity of capto impres media permits high flow velocities. Data file 29067018 ab ion exchange chromatography capto. Pdf data file capto sp impres, capto q impres yumpu. Capto q impres ion exchange chromatography resin cytiva. Capto adhere impres resin is available in the following formats.

Neither ph gradient elution nor nacl gradient elution from capto sp impres chromatography at higher ph. The results show that capto s impact is a useful tool for these challenges faced in todays biopharmaceutical manufacturing. Capto q impres, capto mmc impres, and capto adhere. Hitrap capto sp impres column is prepacked with capto sp impres resin. Pdf effects of salt induced reversible selfassociation on the. Impact 50 m, and capto impres 40 m iex media are summarized in chapter 3. Effects of saltinduced reversible selfassociation on the elution. Approximately 5 ml of q or sp sepharose fast flow were equilibrated in 1 m kcl and titrated with 100 mm. Purification of antibody fragments for the reduction of charge variants using cation exchange chromatography. Highresolution intermediate purification and polishing resin based on the wellestablished capto platform with traditional ligands. Ion exchange chromatography columns and resins ge healthcare. Capto sp impres is a strong cation exchange bioprocess medium, designed to meet the demands of modern largescale manufacturers for fast, efficient, and costeffective intermediate and polishing protein purification. Application capto sp impres is a strong cation exchanger for the highthroughput intermediate purification and polishing steps of a wide range of biomolecules. Pdf some monoclonal antibodies mabs are reported to display concentrationdependent reversible.